Menu
Global B2B sourcing platform
Verified suppliers and manufacturers

Huankai Program Controlled Sealing Machine-Enzyme Substrate Coliform Test Onpg Mug-Elite Tray Sealer

Price $6520.00
Min Order 1 Set
Availability In Stock
Shipping From Guangdong, China
Popularity 525 people viewed
Model NO.:
778860728
After-sales Service:
2 Years
Warranty:
2 Years
Customized:
Non-Customized
Structure:
Desktop
Material:
Plastic
Certification:
ISO
Application:
Lab
Type:
Tray Sealer
Trademark:
EHK
Transport Package:
51X43X51cm
Specification:
51x43x51cm, 22.6kg
Origin:
China
Product Description
Product Description

Set of products:

HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Reagent
HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Sample Vessel
HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Elite-Tray/51 and Elite-Tray/97
HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Elite-Tray Sealer
HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - UV Analyzer

Application:

Used for the principle of enzyme substrate method to detect water coliform, Escherichia coli and fecal coliform
 
How to use

Presence/Absence
  1. Add reagent to a 100mL sample, mix well, and incubate at 36°C ± 1°C for 24 hours.
  2. Read results:
    1. Colorless = negative
    2. Yellow = total coliforms
    3. Yellow/fluorescent = E. coli
Quantification
  1. Add reagent to a 100mL sample and mix well.
  2. Pour the sample into Elite-Tray/51 (counts from 1-200) or Elite-Tray/97 (counts from 1-2,419).
  3. Seal in Elite-Tray Sealer and place in 36°C ± 1°C incubator for 24 hours.
  4. Read results:
    1. Yellow wells = total coliforms
    2. Yellow/fluorescent wells = E. coli
    3. Count positive wells and refer to the MPN table
Principle

The Science


The HuanKai Enzyme Substrate Coliform Test is designed to simultaneously detect total coliforms and E. coli. Two nutrient indicators, ONPG and MUG, are the major sources of carbon in our HuanKai's reagent and can be metabolized by the coliform enzyme β-galactosidase and the E. coli enzyme β-glucuronidase, respectively.
As coliforms grow, they use β-galactosidase to metabolize ONPG and change it from colorless to yellow. E. coli use β-glucuronidase to metabolize MUG and create fluorescence. Since most non-coliforms do not have these enzymes, they are unable to grow and interfere. The few non-coliforms that do have these enzymes are selectively suppressed by HuanKai's specifically formulated matrix.
 

 

Company Information

 

  • Similar Products
Full Page Contact Supplier