3-Aminophthalhydrazide CAS 521-31-3 Luminol Luminescent Reagent Chemical Reagent Lab Equipment

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Hubei New Desheng Material Technology Co., Ltd

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luminol
521-31-3
C8h7n3o2
208-309-4
in Vitro Diagnosis
For Biological Purpose, Pro Analysis
Standard
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
Extractive
Fine Chemicals
Industry, Scientific Research, Health
Biochemical Reagent
Yes
99%
No
Yes
177.16
Alkaline Solution, Dilute Acid
Water and Alcohol
Desheng
Bottle
China
2933990090
Product Description
 

Product Description

 

Luminol, also known as 3-amino benzoyl hydrazine, is a common chemiluminescent substance commonly used in various detection and experiments in the fields of biology and chemistry. Its unique chemiluminescence properties make it widely used in high-sensitivity detection of trace substances and labeling of biomolecular reactions.


Packaging Specification : Dark brown Plastic bottles, 10g/bottle,50g/bottle

 

Product Parameters


  
Product nameLuminolBoiling point1621.9ºC
Molecular formulaC8H7N3O2Water solubility<0.1 g/100 mL (19ºC)
Molecular weight177.16Density1.433 g/cm3
CAS No521-31-3AppearanceLight yellow powder
EIENECS208-309-4Flash point5ºC
Melting point329-332ºCHS code29339990
 

principle

 

The chemiluminescence of Lumino mainly involves two steps: oxidation and proton transfer. In alkaline solution, luminol is oxidized by oxidants (such as hydrogen peroxide, potassium permanganate, etc.) to excited luminol ions (3-amino benzoyl hydrazine cation), which are unstable and release energy and return to the ground state, while emitting photons. During the proton transfer process, the Luminolate ion absorbs protons from the surrounding medium and converts them into a reduced state of Luminolate, completing the cycle.

 


 

Application

 

1. Trace substance detection: The chemiluminescence intensity of luminol is linearly related to the concentration of reactants, making it suitable for the detection of trace substances. For example, in the presence of hydrogen peroxide, luminol reacts with iron ions to generate strong fluorescent substances, which can be used for the detection of trace iron in water samples.

2. Biomolecular markers: The chemiluminescence properties of luminol make it an ideal choice for biomarkers. For example, in immunoassay, luminol is often used to label antibodies or antigens, detecting interactions between biomolecules through chemiluminescence intensity.

3. Environmental monitoring: Luminol can be used for the detection of trace pollutants in environmental samples. For example, in the presence of hydrogen peroxide, Luminol reacts with heavy metal ions to generate fluorescent substances, which can be used for the detection of heavy metal ions in environmental samples such as soil and water.

4. Bioimaging: Luminol can also be used for biological imaging research, such as combining Luminol with fluorescent dyes to label cell or tissue slices, and studying the physiological function of cells or tissues by observing changes in fluorescence signals.

5. Criminal Investigation Blood Stain Detection: Luminol is a chemiluminescent reagent widely used in criminal investigation. At the crime scene, investigators will use luminol to spray on areas that may contain blood, and look for potential evidence by observing the phenomenon of luminescence. This method can not only find blood stains that are difficult to detect with the naked eye, but also establish connections between multiple crime scenes, providing important clues for the investigation of cases.

 

 

Our Advantages



1. The optimal fluorescence wavelength is 425nm (detected in 60mMK2S2O8100mK2CO3, PH11.5 solution)
Learn Luminescence Rate.

2. Luminol/Luminol/Luminol is one of the most commonly used liquid phase chemiluminescence reagents due to its simple structure, easy synthesis, good water solubility, and high luminescence quantum efficiency. Since Albrecht first reported the chemiluminescence reaction of Luminol with oxidants in alkaline solutions in 1928, research on this chemiluminescence system has been very active, making it applied in many fields

 

Company Profile

 

Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!


 



 
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