Audited Supplier
CK-EA1
Scientific Research
Elisa Kit
Only for Research Use
48 or 96t
10UL
99.5%
99.9%
15 Min
5 Boxes
Human and Animals Research Use
Mornmed
Standard Export Packing
Jiangsu, China
Product Description
ELISA REAGENT KIT ONLY FOR RESEARCH USE
For one test kit manual:
Drug Names
Generic Name:Human vitamin D (VD) ELISA Kit.
Purpose
This kit allows for the determination of VD concentrations in Human serum, plasma, tissue homogenates and other biological fluids.
Principle of the assay
The kit assay Human VD level in the sample, use Purified Human VD antibody to coat microtiter plate wells, make solid-phase antibody, then add VD to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VD in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
Detailed Images
