Jak2 V617f Mutation Kit (ARMS-qPCR)

[Intended Use]
The JAK family of non-receptor tyrosine kinases, includes JAK1, JAK2, JAK3, and TYK2. Growth factors and cytokines can regulate gene transcription through JAK-dependent activation of signal transducer and activator of transcription (STAT). The JAK-STAT signal transduction pathway regulates cell proliferation, differentiation, apoptosis and immune regulation. Activation of JAK2 by mutation of the amino acid at position 617 (V617F) is associated with myeloproliferative disorders, including polycythemia vera (mutations found in 90% of cases), essential thrombocythemia (50%) and idiopathic myelofibrosis (50%). JAK2 mutation is the main diagnostic in- dicator of MPD. The Ultrassay JAK2 V617F Mutation Detection Kit is a highly selective and sensitive assay for detecting the V617F mutation in the JAK2 oncogene. Ultrassay's patented technology allows detection of 5% mutant DNA in a background of 99% normal DNA, while ensuring that false negatives are minimized. The pro- cedure is easily adapted for use in high-throughput sample processing.

[Principle] 
The mutation hotspot of JAK2 V617 codon was detected by ARMS-qPCR method. Based on the basic principle of fluorescent PCR, primers and specific probes were designed to detect the targets, and the difference of the number (concentration) of the tested substances in the original samples was determined by analyzing the results of amplification.