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colitech
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1 Year
Disposable
Liquid
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Keep in Cold Storage
Enzyme Substrate Method Detection
100 Times/Box
ODM
in Bags
Shanghai,China
Enzyme Substrate Detection Reagent
7.0~7.6
Lab Test
Haicoer
Electronic
Haicoer
Wood Box
100 Times/Box
Shanghai, China
Product Description
Enzyme Substrate Detection Reagent
Character This product is white or light yellow granules
Clarification degree Colorless or slightly yellow
pH 7.07.6
weight 2.7±0.5g
Storage Long term storage, refrigerating, drying, sealing and avoiding light storage at 4 ~ 8
Term of validity 1 years
Clarification degree Colorless or slightly yellow
pH 7.07.6
weight 2.7±0.5g
Storage Long term storage, refrigerating, drying, sealing and avoiding light storage at 4 ~ 8
Term of validity 1 years
Enzyme Substrate Detection Reagent Working principle
In the water samples containing total coliform bacteria, the target bacteria were cultured in the Minimal Medium 0NPG-MUG medium at 36±1 C. The specific enzyme beta galactosidase produced by the total coliform bacteria can decompose the color source substrate of the Minimal Medium 0NPG-MUG medium, which makes the culture medium yellow; meanwhile, the large intestine in the water sample is in large intestine. Escherichia coli produces a specific beta glucuronase to decompose the fluorescent substrate MUG in Minimal Medium 0NPG-MUG medium and produce characteristic fluorescence. The same principle, the heat coliform group (fecal coliform group) will decompose the color source substrate ONPG in the Minimal Medium 0NPG-MUG medium at 44.5±0.5 C, making the medium yellow.
Packaging specification 200/box
Usage method
Packaging specification 200/box
Usage method
Qualitative detection
Setp 1 Add reagent in 100ml water sample, dissolve it. and Culture 24h at 36±1
Step 2 Read result
Colorless = negative
Yellow = total coliform positive
Yellow = total coliform positive
Yellow + fluorescence = Escherichia coli positive
Quantitative detection
Note: the heat tolerant coliform group (fecal coliform) needs
to be cultured at 44.5± 0.5 24h, and yellow is positive.
Step 2 Read result
Colorless = negative
Yellow = total coliform positive
Yellow = total coliform positive
Yellow + fluorescence = Escherichia coli positive
Quantitative detection
Note: the heat tolerant coliform group (fecal coliform) needs
to be cultured at 44.5± 0.5 24h, and yellow is positive.
Quantitative detection
Step 1 Add reagent in 100ml water sample, dissolve it.
Step 2 Pour into the 51 or 97 detection plate.
Step 3 Use LK sealer to seal the detection plate, and cultivate 24h at 36
Step 4 Results qualitative test was used to check the MPN table count.
Note: the heat tolerance coliform group (fecal coliform group) needs to be cultured at 44.5±0.5 24h, and the Yellow pore pattern is positive, and the corresponding MPN table count is found
Step 2 Pour into the 51 or 97 detection plate.
Step 3 Use LK sealer to seal the detection plate, and cultivate 24h at 36
Step 4 Results qualitative test was used to check the MPN table count.
Note: the heat tolerance coliform group (fecal coliform group) needs to be cultured at 44.5±0.5 24h, and the Yellow pore pattern is positive, and the corresponding MPN table count is found
Q1What is your minimum order quantity?
A1The minimum order quantity is very low, and the minimum order is one set. For customized projects, it depends on different requirements.
Q2Do you offer guarantee for the products?
A2Yes, we offer 1-2 years warranty to our products.
Q3What is the delivrey time after an drder placed?
A3Our standard delivery is 3-5days when we have stock.We will let you know the lead time according to your oder quantity in time.
Q4What is the warranty?
A4The free warranty is one year from the day of Commissioning qualified.
Q5Can you tell me your main customers?
A5Sorry we could not tell you except customer's permission. At the same time,your information also will be protected.
Q6How can i get the after-service?
A6We will be responsible for our products in the valid time.
A1The minimum order quantity is very low, and the minimum order is one set. For customized projects, it depends on different requirements.
Q2Do you offer guarantee for the products?
A2Yes, we offer 1-2 years warranty to our products.
Q3What is the delivrey time after an drder placed?
A3Our standard delivery is 3-5days when we have stock.We will let you know the lead time according to your oder quantity in time.
Q4What is the warranty?
A4The free warranty is one year from the day of Commissioning qualified.
Q5Can you tell me your main customers?
A5Sorry we could not tell you except customer's permission. At the same time,your information also will be protected.
Q6How can i get the after-service?
A6We will be responsible for our products in the valid time.
