Audited Supplier
R511102
Plate,Liquid,Powder
Magen Biotech
Box
50 Preps/Kit
China
3822009090
Product Description
Product Name HiPure DNA/RNA Mini Kit
Cat. No. & Specifications R511102,50Preps/kit
Introduction
The Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through an DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Main Composition
| Product | R511102 |
| Preparation Times | 50 |
| HiPure DNA Mini Column | 50 |
| HiPure RNA Mini Columns | 50 |
| 2ml Collection Tubes | 100 |
| Buffer RLC | 50 ml |
| Buffer DW1 | 30 ml |
| Buffer RW1 | 50 ml |
| Buffer RW2* | 20 ml |
| RNase Free Water | 10 ml |
| Buffer AE | 10 ml |
Storage conditions and Validity
HiPure DNA/RNA Kit components are guaranteed for at least 12 months from the date of purchase when stored at room temperature. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Materials and Equipment to be Supplied by User
1.Dilute Buffer RW2 with 100% ethanol and store at room temperature
2.Microcentrifuge capable of at least 12,000 × g
3.If purifying RNA from cell lines rich in RNases, we recommend adding β-mercaptoethanol (β-ME) to Buffer RLC before use. Add 10µl β-ME per 1ml Buffer RLC. Dispense in a fume hood and wear appropriate protective clothing. Buffer RLC containing β-ME can be stored at room temperature (15-25ºC) for up to 1 month. Alternatively, add 20µl of 2M dithiothreitol (DTT) per 1 ml Buffer RLC. The stock solution of 2 M DTT in water should be prepared fresh or frozen in single-use aliquots. Buffer RLC containing DTT can be stored at room temperature for up to 1 month.
Troubleshooting Guide
A:Clogged HiPure RNA Column
1.Too much starting material: In subsequent preparations, reduce the amount of starting material. It is essential to use the correct amount of starting material.
2.Inefficient disruption and/or homogenization: Disrupting and homogenizing starting materia as RNeasy Mini Kit pages 18-21.
B:RNA does not perform well (e.g. in RT-PCR
1.Salt concentration in eluate too high: Modify the wash step by incubating the column for 5 min at room temperature after adding 500ul of Buffer RW2, then centriufge.
2.Eluate contains residual ethanol: Ensure that the wash flow-through is drained from the collection tube and that the column is then centrifuged at >12,000 x g for 1min.
C:DNA contamination in downstream expeiments
1.No DNase treatment: Perform optional on column DNase digestion using RNase-Free DNase Ste at the point individual protocols.
