Audited Supplier
R480101 / R480102
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Biochemical Reagents
Reagent Grade
For Biological Purpose, For Microbiological, Magzol Reagent
Standard
Laboratory Reagents, Analytical Reagents, Teaching Reagents, Rna Isolation Solvent (Substitution Trizol/Qiazol
Laboratory Production
Magzol Reagent
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Animal Tissues, Cells and Simple Plant Tissues
Biochemical Reagent
Rna Isolation Solvent (Substitution Trizol/Qiazol
Rt-PCR, Northern Hybridization, Poly (a) Enrichmen
Acid Phenol Guanidine Isothiocyanate
Manual (Centrifugation)
Animal Tissue, Plant Tissue, Adherent Cells
Suspended Cells, Bacteria, Trace Fungi
Flexible
Mini Spin Column 96 Well Plate
Variation with Sample Size
Variation with Sample Size
Free Sample
Technical Support
Magen Biotech
Box
100ml / 200ml
China
90279000
Product Description
MagZol Reagent
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R480101 | MagZol Reagent | 100 ML | $79.00 |
| R480102 | MagZol Reagent | 200 ML | $149.00 |
△ Introduction
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi.
The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour.
Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
△ Details
| Features | Specifications |
| Main Functions | RNA isolation solvent (substitution for Trizol/Qiazol reagent) |
| Applications | RT-PCR, Northern hybridization, poly (a) enrichment, etc. |
| Purification technology | Acid phenol guanidine isothiocyanate |
| Process method | Manual (centrifugation) |
| Sample type | Animal tissue, plant tissue, adherent cells, suspended cells, bacteria, trace fungi, etc. |
| Sample amount | Flexible |
| Elution volume | Variation with sample size |
| Time per run | Variation with sample size |
△ Advantages
- Extensive - suitable for various kinds of biological samples, including animals, plants, cultured cells, bacteria, etc.
- High yield - efficient cleavage releases more RNA
- High purity - purified RNA is suitable for various downstream applications
- Flexible - sample amount can be adjusted according to the demand
- High cost performance compared with similar products
△ Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.
Experimental data
