Audited Supplier
C14121 MPG-380
Magen Biotech
Bottle
380ml
China
3822009090
Product Description
Product Name MagPure Particles G
Product specifications C14121,MPG -380mlBinding Mechanism
An appropriate lysis buffer is first added to the sample, followed by incubation with beads. The beads with bound DNA/RNA are easily pulled to the side of the test tube by applying a magnetic field, and unbound material is removed by aspiration. The magnetic separation also facilitates simple washing and elution of the isolated nucleic acids. The protocol and buffer systems used should be optimized relative to your specific sample type and target material. Magnetic-based methods are ideal for automation of high throughput processing, as they eliminate the need for centrifugation and other time-consuming steps.
Description
The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.
Specification
1.Appearance (Form) : brown to deep yellow
2.Form: Amorphous and Porous
3.Powder composition: 60~70% Fe2O3, 0~10% FeO and 30% SiO2
4.Function groups: Si-OH
5.Size: 0.5~2um
6.Dry Materials: 75~80mg/ml.
7.Dispersivity: Monodisperse and Polydisperse Mixture
8.Usage: For 1ml reaction Volume, add 20~30ul MagPure Particle (Liquid Format)
9.Recommend: For Plasmid Isolation and Clean Up. For Viral Nucleic Acid Isolation, less than 10ug genomic DNA Isolation.
Main Composition
| MagPure Particles G | |
| Cat.No. | C14121 MPG-380 |
| Quantity | 380ml |
| Concentration | 40mg/ml |
| Form | Amorphous and Porous |
| Surface function | Si-OH, Silica Beads |
| Dispersion | Polydisperse |
| Particle Size | 2um |
| Color | Brown |
| Magnetic response | Medium |
| Settling Time (1ml) | >4min |
| Usage (0.2ml Sample) | 20-30ul |
| DNA Recover Rate (only 4M GITC) | >90% |
| DNA Recover Rate (10% PEG8000/NaCl) | >80% |
| Recommended Use | 1.Circulating DNA Isolation 2.Viral Nucleic acid Isolation 3.gDNA Isolation 4.FFPE DNA/RNA Isolation |
| Magen Kits | IVD5432, IVD5435 |
Storage conditions and Validity
Room temperature, up to 2 years.
Features
1.Flexible: manual or automated high-throughput DNA/RNA isolation
2.Fast magnetic response: only 3 seconds for complete separation
3.High yields: up to 10 µg of genomic DNA from 200 µL of whole blood
4.Pure: isolated DNA is immediately ready-to-use in downstream application such as sequencing, in vitro transcription, restriction enzyme digestion, and or transfection of robust cell lines
5.Reproducible results and lot to lot consistency: CV < 5%
