Product Name MagPure Blood RNA Kit
Product specifications 48 Preps/KitIntroduction
This product supplies a simple and rapid extraction of total RNA from Blood, buffy coat, bone marrow, Cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process is only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤ 5x106 cells suspension, 200~300 µl Whole Blood, 200µl buffy coat, 100µl bone marrow.
Main Composition
Cat.No. | R661101 |
Purification times | 48 Preps |
MagPure RNA Particles | 1.7 ml |
Proteinase K | 24 mg |
Protease Dissolve Buffer | 1.8 ml |
DNase I | 600 µl |
DNase Buffer | 30 ml |
Buffer MLB | 40 ml |
Buffer MCB | 9 ml |
Buffer MW1 | 22 ml |
Buffer MW2 | 20 ml |
RNase Free Water | 10 ml |
Storage conditions and validity
This product can be stored at room temperature (15~25oC) for 12 months. MagPure RNA Particles, Proteinase K, DNase I are shipped at room temperature. After received, store DNase I at -20ºC and store MagPure RNA Particles and proteinase K at 2~8ºC.
Materials and Equipment to be Supplied by User
KingFisher or similar Extractor isolation:
Name of the Plate | Pre-loaded reagents | Addition before use |
Sample plate |
| |
Wash Plate 1 | 600µl Buffer MW1, Put in 96 magnetic Tip | |
DNase | 290µl DNase Buffer and 10µl DNase I | |
Wash Plate 2 | 600µl Buffer MW2 | |
Wash Plate 3 | 600µl Buffer MW2 | |
Elution plate | 60µl RNase Free Water |
2.Place a 96 tip comb for deep well magnets on Wash Plate 1.
3.Start the R6611_Flex protocol with the KingFisher Flex 96 and load the plates.
Troubleshooting Guide
B:Low A260/A280 value




