Model NO.:

R414302 / R414303

CAS No.:

Formula:

EINECS:

Classification:

Biochemical Reagents

Grade:

Reagent Grade

Specific Usage:

For Biological Purpose, For Microbiological, for Tumor Detection Cancer Screening

Content:

Standard

Usage:

Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation Total Rna From Ffpe Tissue

Source:

Lab Production

Habit Appellation:

Hipure Ffpe Rna Kit

Application:

Industry, Scientific Research, Health, Environmental Protection, Agriculture, Tumor Detection

Property:

Biochemical Reagent

Main Functions:

Isolation Total Rnafrom Ffpe Tissue/Section Sample

Applications1:

Rt-PCR, Quantitative Rt-PCR, Northern Hybridizatio

Applications2:

Poly a Purification, Nucleic Acid Protection

Applications3:

in Vitro Translation

Products:

Tumor Testing, Cancer Screening

Purification Method:

Mini Spin Column

Purification Technology:

Silica Technology

Process Method:

Manual (Centrifugation or Vacuum)

Adaptive Instrument:

Nucleic Acid Extractor, Pipetting Workstation

Sample Type:

Ffpe Tissue Sample, Section Samples

Sample Amount1:

6mg

Yield:

20μg

Elution Volume:

≥20μl

Liquid Carrying Volume Per Column:

800µl

Binding Yield of Column:

100µg

Trademark:

Magen Biotech

Transport Package:

Bottle/ Box

Specification:

50 preps/ 250 preps

Origin:

China

HS Code:

3822009090

Product Description

HiPure FFPE RNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
R414302	HiPure FFPE RNA Kit	50 preps	$149.00
R414303	HiPure FFPE RNA Kit	250 preps	$671.00

△ Introduction

HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.

△ Details

Features	Specifications
Main Functions	Isolation total RNA from FFPE tissue and section samples
Applications	RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	FFPE tissue sample
Sample amount	6mg
Yield	20μg
Elution volume	≥20μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

△ Advantages

High quality - high purity total RNA can be directly used in various sensitive downstream applications
Fast - several samples can be extracted in 60 minutes by column method
Safe - no phenol chloroform extraction required
Sensitive - RNA can be recovered at the level of PG

△ Kit Contents

Contents	R414302	D414303
Purification Times	50 Preps	250 Preps
HiPure RNA Micro Columns	50	250
2ml Collection Tubes	50	250
Buffer DPS	60 ml	250 ml
Buffer FRL	15 ml	60 ml
Buffer RLC	15 ml	60 ml
Buffer RWC*	10 ml	50 ml
Buffer RW2*	20 ml	2 x 50 ml
Protease Dissolve Buffer	1.8 ml	10 ml
Proteinase K	24 mg	120 mg
RNase Free Water	10 ml	20 ml

△ Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

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Hipure Ffpe Rna Kit Formalin-Fixed, Paraffin-Embedded Tissue

Magen Biotechnology (Guangzhou) Co., Ltd.