Audited Supplier
R411102/ R411103
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Biochemical Reagents
Classic
For Biological Purpose, For Microbiological, Isolation Total Rna (Not Include Mirna) From 20mg
Standard
Laboratory Reagents, Analytical Reagents, Teaching Reagents, Rt-PCR, Qrt-PCR, Northern Hybridization, Second Ge
Laboratory Production
Hipure Total Rna Plus Kit
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Rt-PCR, Qrt-PCR, Northern Hybridization, Second Ge
Biochemical Reagent
Rt-PCR, Qrt-PCR, Northern Hybridization, Second Ge
Mini Spin Column
Silica Technology, DNA Filtration Technology
Manual (Centrifugation or Vacuum)
Animal Soft Tissue, Cultured Cells, Lymphocytes, S
Cells:≤1 X 107
Animal Tissue Sample: 1-20 Mg
Plant Tissue: 50-150 Mg
2-100μg
≥50μl
≤25 Minutes (1-24 Samples)
800µl
100µg
Free Sample
Technical Support
Magen Biotech
Box
50 Preps/ 250 preps
China
90279000
Product Description
HiPure Total RNA Plus Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R411102 | HiPure Total RNA Plus Kit | 50 preps | $124.00 |
| R411103 | HiPure Total RNA Plus Kit | 250 preps | $547.00 |
△ Introduction
This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
△ Details
| Features | Specifications |
| Main Functions | Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column) |
| Applications | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Sample amount | Cells:≤1 x 107 Animal tissue sample: 1-20 mg Plant tissue: 50-150 mg |
| Yield | 2-100μg |
| Elution volume | ≥50μl |
| Time per run | ≤25 minutes(1-24 samples) |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
△ Advantages
- Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
- High quality - high purity total RNA can be directly used in various sensitive downstream applications
- Fast - several samples can be extracted in 25 minutes by column method
- Safe - no phenol chloroform extraction required
- Sensitive - RNA can be recovered at the level of PG
△ Kit Contents
| Contents | R411102 | D411103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 12 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
△ Storage and Stability
HiPure Kit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
