Audited Supplier
IVD3102
Plate,Liquid,Powder
Magen Biotech
Box
200 Preps/Kit
China
3822009090
Product Description
| Magen's Nucleic Acid Isolation &Purification Kits Germany in-Vitro Diagnostic registered (98/79/EC) Chinese in vitro diagnostic Reagent Glass I, 20150062) | |||||||
| Function Name | Cat.No. | Quality (Preps) | Price | Nucleic Acid Type | Method | Sample | Applicaton |
| HiPure Universal DNA Kit | IVD3018 | 100 | genomic DNA | Silica-gel Column |
| PCR, Real time PCR NGS Library prepare | |
| HiPure Circulating DNA Kit | IVD3182 | 50 | Circulating DNA | 1~5 ml Plasma, Serum, cerebral effusion, ascites, cell culture supernatants. | PCR, Real time PCR NGS Library prepare | ||
| HiPure Viral DNA/RNA Kit | IVD4173 | 100 | Viral Nucleic Acid | ~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant, other body fluids | Real Time PCR PCR | ||
| HiPure Viral RNA Kit | IVD4175 | 100 | Viral RNA | ~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant , other body fluids | Real time PCR PCR | ||
| MagPure Universal DNA Kit | IVD3102 | 200 | genomic DNA | Magnetic Particles |
| PCR, Real time PCR NGS Library prepare | |
| MagPure Viral DNA/RNA Kit | IVD5412 (Bottle package) | 200 | Viral Nucleic Acid | ~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant, other body fluids | Real Time PCR PCR | ||
| IVD5412-F-96 (Precast for kingFisher Flex) | 96 | ||||||
| IVD5412-TL-96 (Precast for 32 channel Machine) | 96 | ||||||
| MagPure Stool DNA Kit | D6364-400 | 400 | Stool DNA | Isolation Total DNA include microbial DNA from ~150mg Stool Samples. | PCR, Real time PCR NGS Library prepare | ||
Introduction
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from blood, buffy coat, tissue and other samples |
| Applications | Second generation sequencing, PCR, real time PCR, etc. |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Sample amount | Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg |
| Yield | 0.1 - 50μg |
| Elution volume | |
| Time per run |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate.
After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
- High purity - OD 260 / 280 : 1.7- 1.9, OD 260 / 230 : 1.5 - 2.0
- Economy - less than 50% of the price of Qiagen and other imported products
- High yield - most optimal process, ensuring the recovery up to 90%
- Strong processing ability - samples including animal blood, cultured cells, animal tissues, etc.
Kit Contents
| Contents | IVD3102 |
| Purification Times | 200 |
| MagBind Particles | 6.6 ml |
| Proteinase K | 90 mg |
| Protease Dissolve Buffer | 10 ml |
| Rnase A | 40 mg |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD | 20 ml |
| Buffer BW1 | 110 ml |
| Elution Buffer | 30 ml |
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
